I went on a HAB bender last night and ordered lots more components. Then he actually dreamt about them all night :-)
The first ever HAB dream? Unless one of you can better :-)
I have now ordered a batch of non-sterile syringes and tubing, and established a wiring colour scheme for the main circuits (it was getting confusing with 5V, 12V and 3.6V high Ampage components)
I spent some time agonising about incubation temperatures for the TwistDX reagents and in fact if we can't get it to work, we could always test at ambient room temp to start with. Having said that, I have bought:
A professional combined probe and infrared foodgrade thermometer - will try both approaches. I wanted a probe or as small as possible otherwise the thermal mass of the probe ends up setting up the sample temperature and not the other way round!
As it is, the chamber looks like being at least 12mm long to accomodate the probe during testing. The probe hole will be sealed up later.
An IR spot might work - we need an optical window in the incubation chamber anyway for the PicoGreen measurement - but even the smallest spots on various instruments were over a cm, which seems unfeasibly big?
As a curveball, I will also try thermocolour plastic (like those forehead FeverStrips)
For the actual incubation, I will try resistive wire, maybe wound around the incubation. I have also found a small hotplate to sit the chamber on, and also a miniature water heater, and to go with that a miniature immersive water pump! (with water tubing wound around the chamber)
So it could be barheateresque, hotplatesque or central heatingesque!
Will keep you posted!
Oliver de Peyer PhD
Division of Molecular Structure
National Institute for Medical Research
Tel 0208 816 2688
Fax 0208 816 2580
Mobile phone +44 7908 783367